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Cryobiology
and Anhydrobiology
Physical
stresses in cryobiology and anhydrobiology. Much of the cellular
damage produced by environmental freezing is due to freezing-induced,
osmotic dehydration and to the rehydration during thawing. Both dehydration
and rehydration impose mechanical stresses on membranes and macromolecules
and several of the resultant strains have been correlated with cellular
freezing and thawing damage. In collaboration with Gary Bryant of RMIT,
we use NMR and thermodynamic techniques to measure stresses and strains
in model membrane systems and in some suspended cells. For more details,
see Biophysics of cryobiology
and anhydrobiology
Freezing
damage in leaves. Freezing damage limits the range of many plant
species, and the re-establishment of eucalypt forests. In collaboration
with Marilyn Ball of ANU, we study the way ice forms in the leaves of
eucalypts, using infra-red imaging and numerical modelling of radiation,
convection, conduction and nucleation.
Selected
Publications
- Wolfe,
J. (2002) "Cellular Thermodynamics"
Encyclopedia of Life Sciences. http://www.els.net London: Nature
Publishing Group. April 2002.
- Wolfe,
J., Bryant, G. and Koster, K. (2002) ""What is unfreezable water,
how unfreezable is it and how much is there?" Cryo-Letters,
23, 157-166.
- Wolfe,
J. and Bryant, G. (2001) "Cellular cryobiology: thermodynamic
and mechanical effects" International Journal of Refrigeration.
24, 438-450.
- Bryant,
G., K.L. Koster and J. Wolfe. (2001) "Membrane behavior in seeds and
other systems at low water content: the various effects of solutes"
Seed Science Research, 11, 17-25.
- Wolfe,
J. and Bryant, G. (1999) "Freezing, drying and/or
vitrification of membrane-solute-water systems" Cryobiology,
39, 103-129.
http://extra.idealibrary.com/production/cryo/1999/39/2/cryo.1999.2195/2195a.
pdf
- Yoon,
Y. H., Pope, J. and Wolfe, J. (1998) "The effects of solutes on
the freezing properties of and hydration forces in lipid lamellar
phases." Biophys. J., 74, 1949-1965.
- Lutze,
J.L., Roden, J.S., Holly, J.C., Wolfe, J., Egerton, J.J.G. and Ball,
M.C. (1998) "Elevated atmospheric [CO2] promotes frost damage in
evergreen tree seedlings" Plant, Cell and Environment, 21,
631-635.
- Yoon,
Y. H., Pope, J. and Wolfe, J. (1997) "Freezing-induced hydration
forces between phosphatidylcholine bilayers - the effect of osmotic
pressure." Colloids and Surfaces A, 129, 425-434.
- Bryant,
G. and Wolfe, J. (1992) "Interfacial forces in cryobiology
and anhydrobiology" Cryoletters 13, 23-36.
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